This kit contains Quickcycl 60mL, MicroBacter Start XLM 125mL, and MicroBacter Clean 500mL. You will use them in that order. Please read each label. This kit contains live bacteria. Do not use other bacteria simultaneously. It is recommended the you NOT use live sand and particularly do not use dry artificial rock that has been seeded with bacteria. There are no dry bacteria that are beneficial to cycling and they will interfere with the bacteria in this kit. Other bacteria products may interfere also.