biogen should be used in a reactor that provides a flow of water that keeps the beads moving. Bacteria will colonize the beads in 2 - 4 weeks. To accelerate this process use a bacterial supplement such as seed or remediation.

Add 60 mL (1/4 cup) per 265 L (70 US gallons) weekly until a total dose of 60 mL (1/4 cup) per 85 L (23 US gallons) is reached. Slowly increasing the amount will prevent sudden drops in phosphate and nitrate and reduce the risk of a bacterial bloom. As beneficial bacteria consume the beads, add more to maintain the dose. Do not wait until the reactor is empty before adding more. It is important to monitor the reactor and adjust the flow to keep the beads moving. Non-moving beads may result in anaerobic conditions and possible production of hydrogen sulfide. If this happens, remove the beads from the reactor, rinse them, replace the beads and restart the flow. A protein skimmer is recommended to remove any excess bacteria biomass. For a system without a skimmer, a regularly cleaned mechanical filter is adequate.